REWOFUEL aims to convert residual wood into bio-isobutene (bio-IBN) through fermentation, and then convert bio-IBN into advanced, drop-in biofuels and co-products. The process will be scaled up to TRL6-7 and produce renewable and sustainable alternatives to fossil-based isobutene. Co-products include energy-lignin, biogas, microbial-proteins, organic-fertilizers, and bitumen-lignin. The biofuels and co-products produced could save up to 510 kt of CO2 annually and do not require engine modification. Life cycle and sociotechnical assessment was applied by the Energieinstitut to quantify the potential and impact of all steps.